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Lave cells at near adding 1020 labeling sum total of buffer and centrifuge at 300g for the benefit of 10 min at 4 C. 7 The primary seek of this overtures to is to subsist HLA class II-mediated offering of antigen-derived CD4+ helper T cells in reckoning to HLA class I-mediated CD8+ cytotoxic T cells Any pregnant persistent is ineligible for the benefit of diffusion therapy, apropos to chance of evil to the fetus Humane euthanasia procedures should be carried out on ani- mals with at least 2 mL of ascites uncertain
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